Growth factors were synthesized by ovarian somatic cells and they have effect on cumulus cell expansion and oocyte maturation in vitro. Fibroblast growth factor 10 (FGF10) was known as regulating the maturation of mammalian cumulus-oocyte complexes. In this study, we investigated the various gene expression level and effect of the 5, 10, 50 and 100 ng/ml FGF10 (5 F, 10 F, 50 F and 100 F) on cumulus cell expansion and oocyte maturing in vitro. The 10 F treated group was significantly increased in the rate of fully expanded cumulus cell compared to the other groups in MII stage oocytes. In cumulus cell expansion genes, HAS transcript level was significantly increased in all the FGF10 treated groups compared to control group at GVBD stage. In the mRNA expression level of CTSB, the 10 F treated group was remarkably decreased compared to other groups at MII stage. The normal spindle formation at GVBD stage in the 10 F treated group were higher than that of other groups. The MII stage oocyte was increased in 5 F, 10 F and 100 F treated groups compared to control and 50 F treated groups. Relative mRNA expression of GDF9 and BMP15 was significantly higher in the 10 F treated group than other groups at GVBD stage. The BMP15 and CyclinB expression levels were increased in the 10 F treated group compared to all other groups at MII stage. The cleavage, blastocyst formation and total cell number was significantly increased in 10 F treated group compared to all other groups. These results demonstrated that 10 F is efficient to enhance the cumulus cell expansion, oocyte maturation and embryo development.