Introduction

Somatic cell nuclear transfer (SCNT) technique is a key point of producing transgenic animal disease models. During in vitro production of SCNT embryos, the quality of matured oocytes is one of the important factors that regulate embryo developmental capacity.

Materials and Methods

Groups of 50 cumulus oocytes complexes were matured in TCM-199 supplemented with 0 or 10 ng/mL FGF10 (Con and 10 F). Following maturation, the first polar body and nucleus were enucleated. The donor cell was inserted and after fusion, reconstructed embryos were cultured in culture medium.

Results and Discussion

The maturation, cleavage and blastocyst formation rates were higher in the 10 F than Con. The average time of 2- and 4-cell formation was faster in 10 F than Con. In 10 F, total cell number was increased and apoptosis was decreased at day 7. The transcript level of CASP3 decreased and BIRC5, POU5F1 and that of SOX2 increased in the 10 F compared to Con. In this study, we confirmed that 10 F enhances the oocyte maturation and developmental capacity. These results demonstrate that 10 F treatment can be used for in vitro development of porcine SCNT embryos and subsequent production of transgenic animal model.