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[학술발표] ▶2년차◀[국제] WCRB, SG Jeong, Treatment of allicin improves maturation of immature oocytes and subsequent developmental ability of preimplantation embryos, 2017.09.27
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  • 작성일 2020-12-30 17:02:53
  • 조회수 455

Introduction

The efficiency of the in vitro production of oocytes is significantly lower than that of in vivo culture. Allicin (AL) regulates the cellular redox, proliferation, viability, and cell cycle of different cells against extracellular-derived stress. This study investigated the effects of allicin treatment on porcine oocyte maturation and developmental competence.


Materials and Methods

Cumulus oocyte complexes were cultured in IVM medium, containing 0, 0.01, 0.1, 1, 10, and 100

μM AL for 44 h.


Results and Discussion

The rate of polar body emission was tended to be higher in the 0.1 AL-treated group than in the control group (p < 0.1). The rates of cleavage and blastocyst formation significantly increased in the 0.1 AL-treated group (p < 0.05). The rate of phosphorylated p44/42 MAPK to p44/42 MAPK was ~4-fold higher in the 0.1 AL-treated group than in the control group (p < 0.05). The expression of maternal marker (BMP15 and CCNB1) and anti-apoptotic genes significantly increased in the 0.1 AL-treated group at MII stage, respectively (p < 0.05). Consequently, allicin treatment improves porcine oocytes maturation and subsequent development. In conclusion, these results indicate that supplementation of oocyte maturation medium with allicin during IVM improves the maturation of oocytes and the subsequent developmental competence of porcine oocytes.

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