One of the main reasons about low quality of in vitro matured (IVM) oocytes than those of in vivo is the oxidative stress. It caused by external oxygen and incomplete antioxidant system at IVM oocytes. This study was performed to investigate the effects of β-cryptoxanthin (BCX, anti-oxidative reagent) on IVM and further in vitro developmental potential of porcine oocytes and embryos. The oocytes were matured in IVM medium containing 0, 0.1, 1, 10 and 100 μM BCX (control, 0.1, 1, 10 and 100 B, respectively). The oocyte maturation rate was higher in 1 B than in control (p < 0.1), while that of other BCX treated groups were similar to control. After IVM, the cytoplasmic reactive oxygen species (ROS) expression level in 1 B was the lowest among all groups (p < 0.05), while other BCX treated groups were similar to or higher than control. Also, at the classified oocyte maturation stages (GVBD, MⅠ and MⅡ), 1 B significantly decreased ROS and increased GSH level than control (p < 0.05). In addition, the relative mRNA expression level of antioxidant genes, SOD1 and PRDX5, were higher in 1 B than in control (p < 0.05). As the provitamin A, the relative mRNA expression level of retinoic acid receptor genes, RARG and RXRA, were significantly increased in 1 B than in control (p < 0.05). After parthenogenetic activation, the cleavage rates were not different between control and 1 B, however, the blastocyst formation rate was higher in 1 B than in control (p < 0.01). In embryo quality, while the DNA fragmentation of blastocysts was similar between control and 1 B, the total cell number (p < 0.1) and the relative mRNA expression level of pluripotency marker genes, Pou5f1 and CDX2 (p < 0.05), and anti-apoptosis genes, Bcl2L1, Bcl-xl and BIRC5 (p < 0.05), were significantly increased in 1 B than in control. These results demonstrate that BCX is helpful for decreasing oxidative stress in porcine oocytes and improves their quality and developmental potential.