1-1세부

연구성과 1핵심 1-1세부
[SCI] ▶4년차◀ [국제] Antioxidant hesperetin improves the quality of porcine oocytes during aging in vitro, 2018, Molecular Reproduction and Development, 86(1):32-41
  • 글쓴이 관리자
  • 작성일 2020-12-31 14:21:07
  • 조회수 1260

The citrus flavonoid hesperetin has a variety of pharmacological actions, including

antioxidant, antiinflammatory, and anticancer activities. This study investigated whether hesperetin prevents aging of oocytes in vitro in which it determined the

maturation of nuclear and cytoplasm and the developmental capacity of embryo by

modulating the reactive oxygen species (ROS) level. Porcine oocytes were matured in vitro for 44 hr (control) and for an additional 24 hr in the presence of 0, 1, 10, 100, and 250 μM hesperetin (aging, H1, H10, H100, and H250, respectively). Although there was no difference in the rate of maturation among all the groups, both the control and H100 groups significantly increased in the rate of cleavage and blastocyst formation compared to the aging group. The H100 group significantly decreased ROS activity and increases the level of glutathione (GSH) and expression of the antioxidant genes (PRDX5, NFE2L, SOD1, and SOD2) compared with the aging group. The H100 groups prevented aberrant spindle organization and chromosomal misalignment, blocked the decrease in the level of phosphorylatedp44/42 mitogenactivated protein kinase and increased the messenger RNA expression of cytoplasmic maturation factor genes (GDF9, CCNB1, BMP15, and MOS). Subsequently, both the control and H100 groups significantly increased the total cell number and decreased the apoptosis cells at the blastocyst stage compared with aging group. The results indicate that hesperetin improves the quality of porcine oocytes by protecting them against oxidative stress during aging in vitro.


목록





이전글 ▶4년차◀ 2019년 제 22회 제주들불축제
다음글 ▶4년차◀ [국제] Allicin protects porcine oocytes against damage during ag...