Introduction
To
overcome the excess damage on in vitro
matured (IVM) oocytes by external oxygen, we supplemented an antioxidant, β-cryptoxanthin (BCX) to IVM medium.
Materials and Methods
The
immatured oocytes were cultured in IVM medium supplemented with 0, 0.1, 1,
10 and 100 μM BCX (control, 0.1 B, 1 B, 10 B and 100 B). Then, we compare the
intracellular ROS generation, GSH content and antioxidant gene expression of
these oocytes and following embryos.
Results and Discussion
The intracellular ROS concentration was lowest in 1
B treated group among all treated groups. More detail, in classified maturation
stages (GVBD, MⅠ and MⅡ stage), the concentration of ROS decreased and that
of GSH increased in 1 B treated group compared to control group. In addition, the relative mRNA
expression level of antioxidant genes (superoxide dismutase-2 and
peroxiredoxin-5) was significantly higher in 1 B treated
group than
control group. Finally,
the maturation of oocytes and development of embryos in 1 B treated group were
higher than those of control group. In conclusion, β-cryptoxanthin is
helpful for preventing ROS generation of porcine follicular oocytes and
improves their developmental potential.