Introduction

Sperm treatment methods affect sperm fertility and embryo developmental potential in in vitro fertilization (IVF) system and selection of high quality sperm for production of elite Jeju Black Cattle (JBC) zygotes is necessary. In this study, we selected an effective sperm treatment method and superior breeding bull semen.

Materials and Methods

In order to find out the most suitable sperm treatment method, the following methods of percoll gradient, triladyl and percoll-triladyl combination (SF, T and TSF) were used to IVF. To confirm the capacitation, sperm was stained with chlorotetracycline (CTC). We checked the capacity of JBC sperm A, B and C through embryo development after IVF with them.

Results and Discussion

The blastocyst formation rate was increased in T group than SF and TSF groups. The T method can collect only a small amount of sperm, and the SF method is toxic. To compensate for this disadvantage, we combined the T and SF methods and then experimented with the TSF method. As the result of CTC staining, acrosome-reacted sperm (AR pattern) is the highest in sperm C. After IVF, the blastocyst formation rate was the highest in sperm C used group, but the total cell number has no difference. These results demonstrate that sperm C was effective in production of elite IVF embryos.