As wound contraction in the cutaneous layer occurs rapidly in mice, mechanicalmeans are typically used to deliberately expose the wound to properly investigatehealing by secondary intention. Previously, silicon rings and splinting models wereattempted to analyze histological recovery but prevention of surrounding epidermalcell migration and subsequent closure was minimal. Here, we developed an idealchimney wound model to evaluate epidermal regeneration in murine under hESCECtransplantation through histological analysis encompassing the three phases ofregeneration: migration, proliferation, and remodeling. Human embryonic stem cellderived endothelial cells (hESC-EC) were transplanted due to possessing a wellknowntherapeutic effect in angiogenesis which also enhances epidermal repair todepict the process of regeneration. Following a standard 1 mm biopsy punch, achimney manufactured by modifying a 1.7 mL microtube was simply inserted intothe excisional wound to complete the modeling process. Under this model, theexcisional wound remained fully exposed for 14 days and even after 4 weeks, onlya thin transparent layer of epidermal tissue covered the wound site. This approachis able to more accurately depict epidermal repair in relation to histology whilealso being a user-friendly and cost-effective way to mimic human recovery inrodents and evaluate epithelial repair induced by a form of therapy.
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