In mice, wound contraction in the cutaneous layer rapidly occurs. Typically, the wound is deliberately exposed to properly investigate healing by secondary intention. In this study, we developed a new chimney wound model to evaluate epidermal regeneration in murine under human embryonic stem cell-derived endothelial cells (hESC-EC) transplantation through histological analysis encompassing the three phases; migration proliferation, and remodeling of tissue regeneration. Endothelial cells were transplanted due to possessing a well-known therapeutic effect in angiogenesis which also enhances epidermal repair in order to depict the process of regeneration. A chimney was manufactured by modifying a micro tube, which was inserted into the excisional wound to complete the modeling process. The excisional chimney wound remained fully exposed for two weeks and even after one month, only a thin transparent layer of epidermal tissue covered the wound site. Our approach is alble to closely mimic human recovery in rodents for evaluation of treatment.